摘要
目的 观察雷公藤多甙 (MT)体外对成骨细胞 (OB)功能表达的影响 ,以进一步探讨雷公藤 (TW)致女性骨质疏松的机制。方法 分离新生SD大鼠头盖骨OB进行原代培养 ,经 5～ 6d至汇合 ,传代后加入不同浓度MT ,用对硝基苯磷酸法和考马斯亮蓝法作碱性磷酸酶 (ALP)比活性测定 ,茜素红染色法作钙化结节计数测定OB矿化能力。结果 培养 6d ,MT 1μg/ml起显著抑制OBALP比活性 (P <0 0 5 ) ;培养 2周 ,MT 10 -4 μg/ml起显著抑制OB矿化能力 (P <0 0 5 ) ;药物对OB功能表达的抑制程度与其剂量密切相关 (P <0 0 5 ,P <0 0 1)。结论 MT显著抑制OB功能表达且呈剂量依赖性。TW对OB的直接抑制作用 ,是其导致药物性骨质疏松的一个重要原因。
Objective The effects of multiglucoside tripterygii (MT) on the function of osteoblasts were studied in order to further explore the mechanism of osteoporosis induced by Tripterygium wilfordii (TW).Method Osteoblasts were obtained from the calvariae of new born SD rats and were cultured with MT of various concentrations.The effects of MT on alkaline phosphatase (ALP) activity and mineralization ability of osteoblasts were detected.Results 1 μg/ml MT significantly suppressed ALP activity,and 10 4 μg/ml MT inhibited mineralization ability of osteoblasts remarkably.These effects were significantly correlated with MT concentrations.Conclusion It is indicated that MT has direct suppressive effects on the function of osteoblasts, which may play an important role in the pathogenesis of osteoporosis induced by TW.
关键词
雷公藤
雷公藤多甙
成骨细胞
骨质疏松
Tripterygium wilfordii
Multiglucoside tripterygii
Osteoblasts
