蓝色素的发酵条件和特性的研究

The research on the fermentation condition and characteristics of cyanine

对一种蓝色素的发酵条件和特性进行了研究。结果显示,最佳的培养基配方为甘油20g/L,KNO31g/L,K2HPO40.5g/L,NaCl 0.5g/L,MgSO4.7H2O 0.5g/L,FeSO4.7H2O 0.01g/L,pH 8.5;发酵条件为250mL摇瓶装100mL培养基,30℃,150r/min振荡培养96h,发酵液的最高色价为12.6。该色素溶在中性及碱性的水溶液中呈现鲜艳的蓝色;高于60℃时,色素稳定性较差,不宜用于高温处理的食品中;光照对色素稳定性影响较小,保存无需避光;甜味剂、氧化剂及防腐剂等食品添加剂对色素影响较小,而抗坏血酸对色素影响较大。

The fermentation condition and characteristics of Cyanine were studied, The results showed that the best formulaofthe medium was glycerin 20g/L, KNO31g/L, K2HPO40.5g/L, NaC1 0.5g/L, MgSO4·7 H/O 0.5g/L, FeSO4· 7 H20 0. 01g/L, pH 8.5. The fermentation conditions were 100mL culture medium in 250mL shake flask, 30℃, 150r/min shaking 96h, and the highest color value was 12. 6. The pigment dissolved in the neutral and alkaline solution presented the bright blue. More than 60℃, the stability of pigment.was poor. The pigment should nor be plied in the food treated in the high temperature. Light had little effect on the pigment. The food additives, such as sweetener, antioxidants and preservatives had little effects on the pigment, while ascorbic acid affected the pigment more.