普鲁布考等药物对溶血磷脂酰胆碱引起的牛脑基底动脉条收缩的影响

Effects of probucol, etc. on lysophosphatidylcholine-induced vasoconstriction of bovine basilar artery in vitro

目的 :研究溶血磷脂酰胆碱 (L PC)对牛脑基底动脉条收缩的影响及普罗布考等药物的保护作用。方法 :通过描记基底动脉条的张力变化 ,测定 L PC对基底动脉条收缩的影响。结果 :L PC能剂量依赖性 (1× 10 - 9～ 1× 10 - 5 mol/ L )促进基底动脉条收缩 ,在 1× 10 - 5 mol/ L时 ,收缩达最大 ,为 (15 1.0± 18.4) %。当用 Triton X- 10 0 (0 .1% )破坏血管内皮层后 ,L PC诱导的血管收缩作用消失。银杏叶提取物 (EGb)、普罗布考 (Pro)和维生素 E(Vit E)能剂量依赖性地减弱 L PC诱导的牛脑基底动脉条收缩。 结论 :药物 EGb,Pro和 Vit E能拮抗 L PC诱导的牛脑基底动脉条收缩。

Objective: To study inhibitory effects of probucol, etc. on lysophosphatidylcholine(LPC) induced vasoconstriction of bovine basilar arterial in vitro . Methods: Changes in tension of bovine basilar arterial strip were recorded. Results: LPC provoked concentration dependent (between 1×10 -9 1×10 -5 mol/L) contraction of bovine basilar artery. At 1×10 -5 mol/L the effect of LPC reached maximum (151.0±18.4)%. When an endothelial layer was damaged by Triton X 100 (0.1%), the contraction induced by LPC was abolished. Extract ginkgo biloba (EGb), probucol (Pro) and vitamin E (VitE) attenuated contraction induced by LPC in a dose dependent manner. Conclusion: EGb, Pro and VitE may decrease the LPC induced vasoconstriction of bovine basilar artery.