东北部分地区蜱携带几种病原立克次体的分子检测

Molecular detection of rickettsias DNA in ticks around northeast region of China

目的为了解东北地区蜱中携带立克次体的情况。方法应用人工小时布旗法采集东北部分地区不同生境的游离蜱,采用PCR作斑点热立克次体、查菲埃立克体、人粒细胞无形体DNA检测。结果从东北地区全沟硬蜱和森林革蜱检出查菲埃立克体DNA,全沟硬蜱平均阳性率为3.29%,森林革蜱为1.88%;从全沟硬蜱检出人粒细胞无形体DNA,平均阳性率为2.98%;从嗜群血蜱检出斑点热立克次体DNA,其平均阳性率(6.67%)明显高于全沟硬蜱(1.69%)和森林革蜱(2.36%)。结论东北地区全沟硬蜱和森林革蜱可能是查菲埃立克体、人粒细胞无形体的主要携带媒介,嗜群血蜱主要携带斑点热立克次体,提示东北地区存在三种蜱媒立克次体病的自然疫源地。

Objective To understand the prevalence of tick-borne rickettsias around northeast region of Chi-na.Methods Wild ticks were captured and polymerase chain reaction（PCR）was used to amplify the rickettsias DNA of spotted fever group,human granulocytic ehrlichia and Ehrlichia chaffeensis carried by ticks.Results Ehrlichia chaf feensis DNA was found in I.Persulcatus and D.silvarum with the positive rates of 3.29% and 1.88% respectively.Meanwhile,human granulocytic ehrlichia DNA was amplified from I.Persulcatus with the positive rate of 2.95%.However spotted fever group Rickettsiae was mainly found in H.concinna,with as high as 6.67% ticks carrying this rickettsiae,higher than that in I.Persulcatus and D.silvarum which were only 1.69% and 2.36%.Conclusions I.Persulcatus and D.silvarum are possibly the main hosts of human granulocytic ehrlichia and Ehrlichia chaffeensis,while H.concinna mainly carries spotted fever group Rickettsiae in northeast region of China,indicating that it might be the natural foci of spotted feve、human granulocytic anaplasma and Ehrlichiosis.