红腹锦鸡线粒体基因组全序列及其结构分析

Analysis of the Characteristics of Mitochondrial Genomes in Chrysolophus pictus

采用PCR扩增方法测定红腹锦鸡Chrysolophus pictus线粒体基因组全序列:序列全长16 678bp,共有13个蛋白质编码基因、2个rRNA基因和22个tRNA基因。基因组的组成、顺序、编码链的选择、tRNA的结构都与绝大多数鸟类相同或相近。红腹锦鸡线粒体基因组碱基组成分别为:A(30.4%)、T(24.8%)、C(31.2%)、G(13.6%)。总的A+T含量为55.2%。除了COⅠ基因起始密码子是GTG,其余12个蛋白质编码基因的起始密码子都是ATG。tRNA基因核苷酸长度为65～76nt,12S rRNA和16S rRNA基因分别为968和1 604 nt。

The complete mitochondrial genome of Chrysolophus pictus was sequenced by PCR amplification.The entire mtDNA sequence was 16 678 bp in length and contained 13 protein-coding genes,2 rRNA genes,22 tRNA genes,and a control region（D-loop）.Its gene arrangement pattern was identical with those of other avian.The average nucleotide composition was：30.4% of A,24.8% of T,31.2% of C,13.6% of G.All protein-coding genes initiated with ATG,except for COI,which began with GTG.Transfer RNA genes ranged in length from 65 to 76 nt.The 12S rRNA and 16S rRNA genes in C.pictus were 968 and 1 604 nucleotides long.