摘要
目的探讨盐酸戊乙奎醚（长托宁）对癫痫持续状态（SE）后大鼠海马晚期糖基化终末产物受体（RAGE）和核因子KB（NF—KB）的影响。方法SD大鼠随机分为A组（对照组）、B组（模型组）和c组（盐酸戊乙奎醚组），每组8只。应用氯化锂-匹罗卡品制备SE模型。TUNEL法检测大鼠海马细胞凋亡，Westernblot法检测大鼠海马RAGE及NF—KB065的表达改变情况。结果与A组比较，B组大鼠海马RAGE、NF—KBp65水平明显增高，神经元凋亡增加（P〈0．01）；与B组比较，C组大鼠海马RAGE、NF—KBp65水平降低，神经元凋亡减少（P〈0．01）。结论盐酸戊乙奎醚可抑制RAGE—NF—KB通路和减少神经元凋亡，对SE后大鼠海马神经元发挥保护作用。
Objective To investigate the effect of penequinine hydrochloride on the expression of RAGE and NF - kB in rat hippocampal neurons after status epilepticus. Methods Male SD rats were randomly divided into three groups, ie. a control group, a status epilepticus group and a penequinine hydroehloride group （n = 8 each）. The rats abdominal cavity of status epileptieus group were injected lithium - pilecarpine to establish status epileptieus model. The expression of RAGE and nuclear factor kappa B （ NF - KB） p65 in hippoeampal was measured by Western blot. TUNEL assay was used merely to identify apoptotie cells. Results Compared with the control group, the expression of RAGE and NF - KB 1365 was significantly higher in status epilepticus group and apoptotie cells were significant increased （P 〈 0.01 ）. Com- pared with the status epilepticus group, the expression of RAGE and NF - KB p65 was significantly lower and apoptotie cells were decreased in penequinine hydroehloride group （ P 〈 0.01 ）. Conclusion Penequinine hydroehloride shows a protective effects on rat hippocampal neurons after status epilepticus through inhibiting the expression of RAGE and NF - kB.
出处
《徐州医学院学报》
CAS
2012年第11期715-718,共4页
Acta Academiae Medicinae Xuzhou
基金
国家自然科学基金（39970715）
国家自然科学基金（31100801）
国家自然科学基金（81200858）
