摘要
目的：研究ＨＥＲ２／ｎｅｕ原癌基因胞外区片段（ＥＣＤ）ＤＮＡ直接肌肉注射在小鼠体内表达，体液免疫状况，利用小鼠流产模型观察其体内效应。方法：克隆并构建了人及大鼠ＨＥＲ２／ｎｅｕ原癌基因胞外区片段真核表达载体ｐＣＤＮＡ３－Ｘ，采用肌肉直接注射的方法免疫小鼠，分析目的基因在小鼠体内的表达及其诱导的兔疫应答。结果：在质粒ＤＮＡ直接注射部位可检测到目的基因的表达，并诱导了针对ＨＥＲ２／ｎｅｕ的抗体反应；同时诱发小鼠流产，使免疫鼠产仔数减少，可观察到流产结节形成。结论：通过基因免疫，可诱导针对ＨＥＲ２／ｎｅｕ原癌基因产物的有效免疫应答。
Objective: To induce an immune response by intramuscular immunization with HER2/neu oncogene extracellular do- main (ECD) DNA and observe the effect in vivo by an abortion model in mice.Methods: Human and rat HEH2/neu ECD gene were cloned and inserted into expressive vector pCDNA3. The DNA immunization, expression of HER2/neu ECD, antibody de- tection and abortion in immunized mice were studied. Results: HER2/neu ECD protein expressed on muscle cells in the injected site, specific antibody to HER2/neu was induced by DNA immunization. The average of the offspring per delivery from immu- nized mice was significantly lower than that from control group, and the abortive embryos were found in uterus from immunized mice.Conclusion: The results show that both human and rat HER2/neu ECD gene can induced effective immune response by DNA immunization.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2000年第1期15-17,共3页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助!(39870707)课题
