抗APO-1单抗诱导人膀胱癌多药耐药细胞凋亡的研究

Apoptosis of Human Bladder Cancer BIU-87/ADM Cells Induced by Anti-APO-1 Antibody

目的：探讨人膀胱癌多药耐药细胞凋亡诱导途径。方法：应用免疫细胞化学、原位ＤＮＡ末端转移酶标记法、相差显微镜及电镜技术，观察ＢＩＵ－８７／ＡＤＭ细胞表面Ｆａｓ抗原的表达和抗ＡＰＯ－１单抗对该细胞存活的影响及其特征。结果： Ｆａｓ抗原在ＢＩＵ－８７／ＡＤＭ中有高表达，经抗ＡＰＯ－１单抗作用后，ＢＩＵ－８７／ＡＤＭ细胞存活率明显降低，光镜及电镜下观察到凋亡细胞的形态学改变，原位ＤＮＡ末端转移酶标记阳性。结论：抗ＡＰＯ－１单抗能与肿瘤细胞表面的Ｆａｓ抗原结合，触发人膀胱癌多药耐药细胞凋亡。

Objective: To explore the methods of inducing apoptosis in human bladdel cancer multidrug resistance cells. Meth- ods: Expression of Fas antigen was detected with immunocytochemistry on BIU-87/ADM cells, and the effect of anti-APO-1 anti- body was examined with in situ DNA terminal deoxynucleotydyl transferase assay (TDT), phase-contrast and electron microscope techniques. Results: We found that Fas antigen was highly expressed on BIU-87/ADM cell surface, and after treatment with an- ti-APO-1 antibody, survival rate of BIU-87/ADM cells was decreased, apoptotic morphologica changes and TDT positive cells were found. Conclusion: These results suggested that anti-APO-1 antibody could induce apoptosis of BIU-87/ADM cells by combining FAS antigen.