高效液相色谱法测定青风藤水提物缓释片中青藤碱含量

Determination of Sinomenine in Caulis Aqueous Extract Sustained Release Tablets by HPLC

目的建立青风藤水提物缓释片中青藤碱的含量测定方法。方法采用HPLC法,色谱柱:kromasil C18柱(250 mm×4.6mm,5μm);流动相:甲醇-磷酸盐缓冲液(55∶45);流速:1.0mL/min,紫外检测波长:262nm,柱温:25℃。结果青藤碱的线性范围为0.451-2.730 mg/mL(r=0.9999);加样回收率为97.83%(RSD=1.4%)。结论该方法操作简便,结果准确,重复性好,可作为青风藤水提物缓释片的质量控制方法。

Objective Establish Caulis water to mention the matter of sustained release tablets of sinomenine method for the determination.Methods HPLC method was used.Column：kromasil C18（250mm×4.6mm,5μm）.mobile phase：Methanol-phosphate buffer（55∶ 45）.The flow rate：1.0mL/min.The detection wavelength：262nm.The column temperature：25℃.Results The sinomenine linear range of 0.451-2.73 mg/mL（r=0.9999）.Recovery was 97.83%（RSD=1.4%）.Conclusion The method is simple.Results were accurate.And good reproducibility.This can be used as Caulis water to mention the matter of quality control methods of sustained-release tablets.