肿瘤组织中转化/转录域相关蛋白TRRAP点突变检测方法

A simple detect method for a point mutation of transformation/transcription domain-associated protein

转录因子TRRAP是维系生命体正常活动的重要蛋白,目前对其功能作用还知之甚少,相关研究方兴未艾.2011年发现TRRAP一个多发点突变与肿瘤相关,该突变亟须在人群样品中进行检测,以增加对该分子与肿瘤发生关系的了解.本文设计了一个通过PCR扩增,然后酶切的检测方法,有助于了解肿瘤的发生机理.

Transformation/ Transcription Domain-associated Protein; TRRAP, an important transcription factor, was found in 1998 by McMahon, it is still not very clear about its function and its company proteins to date. 2011, Wei found a recurrent point mutation in conserved site of TRRAP by exome sequencing. Here was a detect method for this specific point mutation by PCR and nuclear restriction enzyme digest, through detecting the re- currence mutation situation on tumor patients samples, we will get more comprehensions for this important transcription factor.