牛18ku-bFGF基因真核表达载体构建与鉴定

Construction and identification of eukaryotic expressional vector of bovine 18ku-bFGF gene

将牛18ku-bFGF基因全编码序列插入质粒pEGFP-N1中,构建真核表达载体pCMV-bFGF,并用菌落PCR、酶切和测序等方法对重组质粒进行了鉴定。研究结果表明,检测结果均一致于预期,表明牛18ku-bFGF基因全编码序列已正确插入到载体中。

To further investigate functions of basic fibroblast growth factor（bFGF）,bovine 18ku-bFGF gene was inserted into plasmid pEGFP-N1 to construct eukaryotic expressional vector pCMV-bFGF.The recombinant DNA was identified with colony polymerase chain reaction,digestion with restriction endonucleases and DNA sequencing to be a right insertion of the target gene into the digested plasmid pEGFP-N1 with the two same restriction endonucleases,which indicated that recombinant eukaryotic expressional vector was constructed successfully.