乙型肝炎病毒表面大蛋白的检测及其临床应用

Detection of HBV large surface protein and its clinical significance in patients with hepatitis B

目的检测乙型肝炎病毒表面大蛋白(hepatitis B virus large surface protein,LHBs),并与乙型肝炎病毒脱氧核糖核酸(HBV DNA)相比较,探讨LHBs的临床应用价值。方法分别采用ELISA和PCR法检测乙型肝炎257例血清中LHBs,乙型肝炎病毒标志物(HBV-M)和HBV DNA,并进行相关性分析。结果 LHBs与HBV DNA的检出率差异无统计学意义(P>0.05);LHBs的阳性率(83.12%)高于乙肝e抗原(HBeAg)的阳性率(54.63%),差异有统计学意义(P<0.05)。结论血清中LHBs水平能反映HBV感染者体内的病毒复制情况,将其作为HBV复制指标,灵敏度高于HBeAg,可作为反映HBV感染者体内的病毒复制情况的新的血清学指标,尤其是针对HBeAg阴性患者。

Objective To explore the clinical significance of Hepatitis B virus large surface protein （LHBs） by detecting LHBs. Methods The LHBs were detected by using enzyme linked immunosorbent assay （ELISA）. Surum HBV DNA was detected by using real-time polymerase chain reaction （RT-PCR） and ELISA was used to detect the HBV markers in 257 serum samples from hepatitis B patients. Results No significant difference was found between the positive rate of LHBs and that of HBV DNA（ P 〉 0.05 ）. The positive rate of LHBs was 83.12% ,which was higher than that of HBeAg which was 54.63%. There was significant diference （ P 〈 O. 05 ）. Conclusion The level of serum LHBs can be used to estimate the state of HBV replication and the sensitivity was superior to HBeAg. It may be used as a new serological maker to detect HBV replication, especially in HBeAg negative patients.