两步树脂法从甜叶菊水提液中纯化甜菊糖苷

Two-step Resin Adsorption for Purification of Stevia Glycosides from Stevia Rebaudiana Aqueous Extract

采用两步树脂法从甜叶菊水提液中提取纯化甜菊糖苷。第一步主要是脱色,通过比较大孔树脂D293、D296、JN-1、JN-2、D890和D201处理甜叶菊水提液的脱色率、甜菊糖苷保留率和解吸性能,发现JN-1的效果最好。在优化条件下,甜叶菊水提液经JN-1处理,甜菊糖苷纯度由原来的42.65%提高到67.33%,收率达到84.87%;第二步的主要目的是提高产品纯度,通过比较大孔树脂AB-8、JNF-1、X-5、NKA-II和D101对甜菊糖苷的吸附-解吸性能和得到的纯化液的甜菊糖苷纯度,发现JNF-1的效果最好。在优化的条件下,JN-1处理后的甜菊糖苷溶液进一步采用JNF-1吸附,溶液的甜菊糖苷纯度提高至92.92%,收率91.08%。

A new method for purification of steviol glycosides from Stevia Rebaudiana crude extracts by macro- porous adsorption resin （MAR） was systematically investigated. The purification procedure consisted of two steps. In the first step, the main purpose was decolorization, and several types of macroporous resins including D293, D296, JN-1, JN-2, D890 and D201 were used in order to choose the best resin. JN-1 resin was selected according to their decolorization ratio, the retention of steviol glycosides ratio and desorption ratio. In order to increase the purity, a sec- ond step of refining was added by screening various types of macroporous resins （AB-8, JNF-1, X-5, NKA-II and D101）. The results demonstrated that JNF-1 resin was the most suitable resin. The purity of steviol glycosides after the first step JN-1 was from 42.65% to 67.33% with a recovery of 84.87%. The purity of steviol glycosides in- creased to 92.92% with a recovery of 91.08% after the second step JNF-1.