绞股蓝热处理产物中活性成分的纯化与鉴定及其对A549细胞的抑制活性

Purification, Identification and Anti-A549 Cell Activity of Gypenosides from Thermally Processed Gynostemma pentaphyllum Leaves

目的:提取、分离、鉴定绞股蓝热处理产物中抑制肺癌A549细胞的活性成分。方法:绞股蓝叶在温度125℃、压力0.24MPa条件下,加热处理3h,用80%乙醇加热回流提取3h,利用活性跟踪方法,通过大孔树脂HP-20及反相柱等分离手段,分离抑制肺癌A549细胞的活性成分,并用核磁共振波普(1H-NMR、13C-NMR)、电喷雾质谱(ESI-MS)等数据鉴定有效成分。结果:绞股蓝热处理产物中分离得到2个达玛烷类皂苷,分别为绞股蓝皂苷Gypenoside L和Gypenoside LI,而且它们对肺癌A549细胞具有质量浓度依赖性的抑制活性(P<0.05),其IC50值分别为(48.43±1.33)μg/mL和(34.35±0.88)μg/mL。结论:热处理绞股蓝产物可提高对肺癌A549细胞的抑制作用,其中有效成分为达玛烷类皂苷Gypenoside L和Gypenoside LI。

Objective： To extract,separate and identify anti-A549 cell constituents from thermally processed Gynostemma pentaphyllum leaves.Methods： Gynostemma pentaphyllum leaves were processed at 125 ℃ and 0.24 MPa for 3 h and subjected to heat reflux extraction with 80% ethanol for 3 h.Anti-A549 cell constituents in the extract were separated using HP-20 macroporous adsorption resin and a reversed-phase column by means of bioassay-guided separation and identified by1H-NMR,13C-NMR and ESI-MS.Results： Two dammarane-type saponins were obtained and identified as gypenoside L and gypenoside LI,respectively.Both gypenosides had a concentration-dependent inhibitory effect on A549 cells with IC50 values of（48.43 ± 1.33） μg/mL and（34.35 ± 0.88） μg/mL,respectively.Conclusion： Thermally processed Gynostemma pentaphyllum leaves have a notable inhibitory effect on A549 cells,which is associated with the presence of gypenoside L and gypenoside LI.