猕猴桃籽油的体外抗氧化活性

in vitro Antioxidant Activity of Kiwifruit Seed Oil

以猕猴桃籽油为原料,采用体外抗氧化实验模型研究其抗氧化活性。通过测定猕猴桃籽油还原力和DPPH自由基、超氧阴离子自由基(O2-·)、H2O2以及羟自由基(·OH)清除实验,以VC为阳性对照,探讨猕猴桃籽油的抗氧化性能。结果表明:猕猴桃籽油的还原能力随其质量浓度的增加而增强,对DPPH自由基、O2-·、H2O2以及·OH的清除率随质量浓度的增加而升高,其中对O2-·的清除效果比VC更明显,对其他自由基的清除效果却比VC弱;猕猴桃籽油对O2-·、H2O2、·OH的IC50分别为0.078、0.1、0.2mg/mL,表明猕猴桃籽油清除O2-·的效果最好。

The in vitro antioxidant activity of kiwifruit seed oil was evaluated by reducing power assay and DPPH radical,superoxide anion radical,hydrogen peroxide and hydroxyl radical scavenging assays and compared with vitamin C as a positive control.The results showed that the reducing power and scavenging activity against DPPH radicals,superoxide anion radicals,hydrogen peroxide and hydroxyl radicals of kiwifruit seed oil were directly proportional to concentration.Kiwifruit seed oil could scavenge superoxide anion radicals better than vitamin C but was less effective in scavenging DPPH radicals,hydrogen peroxide and hydroxyl radicals.The IC50 values against superoxide anion radicals,hydrogen peroxide and hydroxyl radicals were 0.078,0.1 mg/mL and 0.2 mg/mL,respectively.Therefore,kiwifruit seed oil had the strongest scavenging effect against superoxide anion radicals.