蛹拟青霉发酵液中抑菌活性成分的分离纯化

Isolation and Purification of Antibacterial Components in Fermentation Broth of Paecilomyces militaris

以大肠杆菌、白色假丝酵母、黑曲霉为指示菌株,对蛹拟青霉xzcs005菌株液体发酵液乙酸乙酯萃取相中的抑菌活性成分进行分离纯化。乙酸乙酯萃取相经硅胶柱色谱分离后得到组分Ⅴ和组分Ⅶ两个抑菌活性组分。组分Ⅴ利用反相柱分离得到抑菌活性纯品PMBA-1,其在质量浓度为1.0mg/mL时对大肠杆菌、白色假丝酵母和黑曲霉的抑菌圈直径分别为(16.21±0.27)、(23.54±0.35)、(27.33±0.54)mm;组分Ⅶ再次经过硅胶柱色谱分离后得到抑菌活性纯品PMBA-2,其在质量浓度为1.0mg/mL时对大肠杆菌、白色假丝酵母和黑曲霉的抑菌圈直径分别为(21.84±0.28)、(16.33±0.54)、(11.54±0.51)mm。

Escherichia coli,Canidia albicans and Aspergillus niger were used as indicator strains for the bioassay-guided separation of antibacterial components from the ethyl acetate extract of fermentation broth of Paecilomyces militaris.The ethyl acetate extract was separated by silica gel column chromatography into two antibacterial fractions,Ⅴ and Ⅶ.Fraction Ⅴ was further separated by reversed-phase column chromatography to obtain a pure antibacterial compound,PMBA-1.At a concentration of 1.0 mg/mL,PMBA-1 presented an inhibition circle diameter of（16.21 ± 0.27） mm,（23.54 ± 0.35） mm and（27.33 ± 0.54） mm against Escherichia coli,Canidia albicans and Aspergillus niger,respectively.Another pure antibacterial compound,PMBA-2,was obtained from further separation of fraction Ⅶ by silica gel column chromatography.Its inhibition circle diameter at a concentration of 1.0 mg/mL was（21.84 ± 0.28）,（16.33 ± 0.54） mm and（11.54 ± 0.51） mm for Escherichia coli,Canidia albicans and Aspergillus niger,respectively.