摘要
从盐生海芦笋中分离出一株内生真菌(Salicorn 15),采用分子生物学与形态学观察相结合对其鉴定。提取该菌的基因组DNA后分别对其18S rDNA和ITS区域进行PCR扩增并进行单克隆测序分析,在NCBI上进行同源性分析,进而构建系统发育树,再结合形态学观察,将菌株Salicorn 15鉴定为层出镰刀菌Fusarium proliferatum。
An endophytic fungus designated as Salicorn 15 was isolated from halophilic Salicornia bigelovii and identified by molecular biology techniques combined with morphological observation.Genomic DNA from Salicorn 15 was extracted and its 18S rDNA and ITS region were amplified by PCR and sequenced for sequence homology analysis using NCBI BLAST.Based on phylogenetic analysis combined with morphological observation,Salicorn 15 was identified as Fusarium proliferatum.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第23期180-183,共4页
Food Science
基金
国家自然科学基金面上项目(31071586)
2011年国家大学生创新训练计划项目(111030739)
江苏高校优势学科建设工程项目
