产α-葡萄糖苷酶抑制剂菌株的筛选及发酵条件优化

Screening of α-Glucosidase Inhibitor-producing Strain and Optimization of Fermentation Conditions

从农田土壤中筛选得到一株产α-葡萄糖苷酶抑制剂能力较强的UN-8菌株,菌株对其发酵条件进行初步优化,得到最优条件为玉米粉3g/100mL、酵母膏1.2g/100mL、溶氧量30mL/250mL、摇床转速160r/min、发酵时间3d、初始pH8.0以及培养温度32℃。在此条件下,α-葡萄糖苷酶抑制剂的抑制率为75.34%,比初始抑制率(60.79%)提高了14.55%。

A high-yield α-glucosidase inhibitor-producing strain named as UN-8 was isolated from farm soil and fermentation conditions for the production of α-glucosidase inhibitor by UN-8 were optimized.The optimal fermentation conditions were determined as follows： 3 g/100 mL of corn meal,1.2 g/100 mL of yeast extract,30 mL of fermentation medium in a 250-mL shake flask,160 r/min of shaking speed,initial pH 8.0 and 32 ℃ of fermentation temperature.The inhibitory rate of α-glucosidase inhibitor produced under these conditions was 75.34%,which was 14.55% higher than before the optimization（60.79%）.