黑灵芝多糖对免疫抑制小鼠的免疫调节和抗氧化作用

Immune Modulation and Antioxidation Activity of Polysaccharides from Ganoderma atrum in Immunosuppressed Mice

目的:研究黑灵芝多糖对免疫抑制小鼠的免疫调节和抗氧化作用。方法:构建免疫抑制小鼠模型,将小鼠随机分为6组,即空白对照组、阳性对照组、模型对照组及黑灵芝多糖高、中、低剂量组。小鼠以灌胃的方式给予不同剂量黑灵芝多糖后,分别测定各组脾脏指数、脾组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)和总抗氧化能力(T-AOC)水平,MTT法测T、B淋巴细胞增殖,ELISA法测脾细胞上清TNF-α和IL-2含量。结果:与模型组相比,黑灵芝多糖各剂量组均能显著提高小鼠脾脏指数,T、B淋巴细胞增殖,提升SOD、CAT、T-AOC水平和降低MDA含量;高、中剂量组能显著提高TNF-α含量和IL-2含量。结论:黑灵芝多糖能有效拮抗环磷酰胺对小鼠免疫功能的抑制作用,提高免疫抑制小鼠的抗氧化功能。

Objective： To study the immunomodulatory and antioxidant effect of polysaccharides from Ganoderma atrum in immunosuppressed mice.Methods： Immunosuppressed mouse models were established by intrapefitoneal injection of cyclophosphamid.Forty-eight mice were randomly divided into 6 groups： blank control,positive control,model control,and high-,medium-and low-dose Ganoderma atrum polysaccharide groups.Ganoderma atrum polysaccharides were administrated by gavage at doses of 100,50 mg/（kg.d） and 25 mg/（kg.d） to mice for 7 consecutive days,respectively.At 24 h after the last administration,the mice in each group was sacrificed and spleens were collected for the measurement of spleen index and the levels of spleen super-oxide dismutase（SOD）,catalase（CAT）,maleic dialdehyd（MDA） and total antioxidant capacity（T-AOC）.Moreover,T and B lymphocyte proliferation was analyzed by MTT and the levels of TNF-α and IL-2 in spleen homogenate supernatant were assayed by ELISA.Results： Compared to control mice,Ganoderma atrum polysaccharides significantly improved mouse spleen index,promoted T and B lymphocyte proliferation,increased SOD,CAT and T-AOC levels,and reduced MDA content in mouse spleen,while only high and medium-dose groups indicated obviously improved TNF-α and IL-2 levels in spleen cells.Conclusions： Ganoderma atrum polysaccharides can effectively antagonize cyclophosphamideinduced immune suppression in mice and enhance antioxidant function in immunosuppressed mice.