芝麻素防治非酒精性脂肪肝作用及机理

Preventive and Curative Effects and Mechanism of Sesamin against Nonalcoholic Fatty Liver Disease in Mice

研究芝麻素(sesamin)对非酒精性脂肪肝病(nonalcoholic fatty liver disease,NAFLD)小鼠的防治作用及其机理。采用高脂饮食建立小鼠非酒精性脂肪肝模型,从血脂水平及游离脂肪酸组成、氧自由基代谢及肝细胞损伤进行研究。结果表明:芝麻素预防剂量组(50mg/(kg.d))、治疗剂量(50、100mg/(kg.d))可有效降低NAFLD小鼠血清总胆固醇(T-CHO)、甘油三酯(TG)水平,显著改善高密度脂蛋白胆固醇(HDL-C)水平;提高NAFLD小鼠血清游离不饱和脂肪酸比例,对调节血脂水平有积极作用。芝麻素还能降低NAFLD小鼠肝脏组织丙二醛(MDA)含量,提高肝脏总抗氧化能力(T-AOC),降低血清谷草转氨酶(GOT)和谷丙转氨酶(GPT)活力。肝脏病理组织学观察表明芝麻素能明显减轻NAFLD小鼠肝脏脂肪化病变的程度。因此,芝麻素对非酒精性脂肪肝病具有防治作用,作用机理与降血脂、调节血脂组成即提高游离不饱和脂肪酸比例、抗氧化和抑制肝细胞损伤有关。

In this study,the preventive and curative effect of sesamin against nonalcoholic fatty liver disease（NAFLD） in mice and its mechanism of action were investigated.NAFLD mouse models were established by feeding high fat diet.Serum lipid and free fatty acid composition,oxygen radical metabolism and liver injury were analyzed to evaluate the preventive and curative effect of sesamin against NAFLD in mice.Sesamin prevention（50 mg/（kg.d）） and treatment（50,100 mg/（kg.d）） effectively decreased serum levels of T-CHO and TG in NAFLD mice,significantly improved serum HDL-C level,increased serum percentage of free unsaturated fatty acids and had positive regulatory effects on serum lipid levels.In addition,sesamin also could lower liver MDA level and serum levels of GOT and GPT and increase liver T-AOC level.Histopathological observation indicated that sesamin markedly alleviated liver lesions in NAFLD mice.Hence,sesamin has both preventive and curative effects against NAFLD in mice and its mechanism of action is relevant to lowering serum lipid levels,regulating serum free fatty acid composition（elevating unsaturated fatty acid proportion）,antioxidation and attenuating hepatocyte damage.