三氯氧磷化学改性玉米抗性淀粉的安全性

Safety Evaluation of Chemically Modified Maize Resistant Starch Prepared with POCl_3

为了检测长期食用三氯氧磷化学改性的玉米抗性淀粉是否给机体带来不良影响和确定其安全用量,首先进行急性毒理学实验,即分4个梯度,灌喂小鼠,观察小鼠的状态,并用加权机率单位法(Bliss法)测三氯氧磷化学改性玉米抗性淀粉的半数致死量(LD50)和95%的可信限;再根据急性毒理学实验的结果,设计90%LD50、75%LD50、50%LD50、25%LD504个梯度,进行为期90d的亚慢性毒理学实验。结果表明:三氯氧磷化学改性玉米抗性淀粉的LD50为33.9g/kg,95%的可信限为23.7～48.3g/kg,则该物质属于实际无毒;亚慢性毒理学实验显示正常人(65kg)的每天摄入量应该小于16526.25mg,即推荐摄入量(RNI)≤16.526g/d。

In order to evaluate the safety of long-term consumption of chemically modified maize resistant starch prepared with POCl3,acute toxicity test was used to determine its safety dosage.For this,the modified starch was given to mice by gavage at four different doses,and median lethal dose（LD50） and 95% fiducial limits（95% FL） were calculated by Bliss method.According to the results of acute toxicology test,4 gradients 90% LD50,75% LD50,50% LD50,and 25% LD50 were designed for a 90-day subchronic toxicity test.The results showed that the LD50of the modified starch was 33.9 g/kg and the 95% FL was 23.7 – 48.3 g/kg,indicating that this material was non-toxic.Moreover,its acceptable daily intake for a normal person（65 kg） should be less than 16526.25 mg,which means that the recommended intake（RNI） is no more than 16.526 g/d.