气相色谱法测定薄荷素油中薄荷脑、薄荷酮、薄荷脑乙酸酯、柠檬烯的含量

Determination of menthol,menthone,menthyl acetate and limonene in Pepermint Oil by gas chromatography

目的采用高效毛细管柱气相色谱法同时测定薄荷素油中主要成分薄荷脑、薄荷酮、薄荷脑乙酸酯、柠檬烯含量。方法色谱柱:HP-FFAP毛细管柱(25m×0.20mm×0.33μm),60℃～230℃程序升温,内标物环己酮。结果各组分线性关系良好,相关系数为1.0000。加样回收率:薄荷脑97.71%,薄荷酮102.18%,薄荷脑乙酸酯98.20%,柠檬烯97.30%。结论该方法简便、准确、可靠,可作为控制薄荷素油质量的方法。

OBJECTIVE Establish a method for the simultaneous separation and determination of menthol,menthone,menthyl acetate and limonene in Pepermint Oil by high performance capillary gas chromatography.METHODS Capillary column HP-FFAP（25m×0.20mm×0.33μm） was used.Temperature programming was from 60℃ to 230℃.Internal standard was Cyclohexanone.CONCLUSION The internal standard menthod showed good linearity（r=1）.The average recoveries were menthol（98.07%）,menthone（102.51%）,menthyl acetate（98.20%） and limonene（97.63%）.RESULTS This menthod was simple,rapid and accurate.It can be used as a menthod of quality control.