正交试验法优选云南松松塔总黄酮的提取工艺

Optimization of Extraction Technology of Total Flavonoids from Pinecone of Pinus yunnanensis by Orthogonal Test

目的:优选云南松松塔总黄酮的提取工艺。方法:采用超声波辅助法提取云南松松塔总黄酮,以总黄酮提取率为指标,考察料液比、提取温度、乙醇体积分数和提取时间对总黄酮提取率的影响,通过正交试验法优选总黄酮的提取工艺。结果:最佳提取工艺为加30倍量60%乙醇于60℃超声辅助提取30 min,云南松松塔总黄酮质量分数3.23%。结论:优选的提取工艺省时、稳定、提取率高。

Objective： To optimize extraction technology of total flavonoids in pinecone of Pinus yunrtanensis. Method： Total flavonoids in pinecone of P. yunnanensis were extracted by ultrasonic assisted extraction method, with extraction rate of total flavonoids as index, orthogonal test was applied to investigate influence of four factors （including ratio of material-liquid, ultrasonic extraction temperature, ethanol concentration and extraction time） on extraction technology of total flavonoids from pinecone of P. yunnanensis. Result： Optimum extraction technology was as following： ultrasonic assisted extracted 30 min with 30 times the amount of 60% ethanol at 60 ℃ , the content of total flavonoids in pinecone of P. yunnanensis was 3.23%. Conclusion： Optimized extraction technology was simple and stable with high extraction rate of total flavonoids.