流苏金石斛中牡荆素2″-O-β-D-葡萄糖苷的分离鉴定及含量测定

Isolation,Identification and Quantitative Analysis of Vitexin 2″-O-β-D-glucopyranoside in the Whole Herb of Flickingeria fimbriata

目的:分离制备流苏金石斛药材指标性成分,并建立测定流苏金石斛中该成分含量的高效液相色谱法。方法:采用Shiseido(Pak-C18)(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水(20∶80)为流动相,流速1.0 mL·min-1,检测波长340 nm,柱温25℃。结果:从流苏金石斛中分离制备其指标性成分牡荆素2″-O-β-D-葡萄糖苷;该成分在0.016～1.6μg线性关系良好(r=0.999 8),平均加样回收率为99.66%(RSD 1.05%)。结论:方法快速简便,可靠,重复性好,为流苏金石斛药材的质量控制提供依据。

Objective： To prepare chemical marker and establish HPLC method for determining this marker in the whole herb of Flickingeria fimbriata. Method： A Pak-C18 （4.6 mm × 250 mm, 5 μm） column was adopted with a mobile phase of acetonitrile-water （20： 80） at the flow rate of 1.0 mL ·min^-1and the column temperature was set at 25 ℃. The detection wavelength was 340 nm. Result： Vitexin 2″-O-β-D-glucopyranoside was firstly isolated as a chemical marker from F. fimbriata. This marker showed a good linear relationship in the range of 0. 016-1.6 μg （r =0. 999 8） and the average recovery was 99.66% （RSD 1.05% ）. Conclusion： The method is quick, simple, reliable, and reproducible, which can be used for quality control of F. fimbriata.