HPLC-ELSD同时测定枇杷叶中6种三萜酸成分的含量

Simultaneous Determination of Content of Six Triterpene Acids in Eriobotrya japonica by HPLC-ELSD

目的:建立HPLC-ELSD同时测定枇杷叶中6种三萜酸成分含量的方法。方法:采用Ultimate XB C18色谱柱(4.6mm×250 mm,5μm),流动相乙腈-甲醇-0.5%醋酸铵溶液,梯度洗脱,流速1.0 mL·min-1,柱温35℃,蒸发光散射检测器漂移管温度95℃,氮气流速2.5 L·min-1。结果:蔷薇酸、委陵菜酸、马斯里酸、科罗索酸、齐墩果酸、熊果酸分别在0.436～2.180,0.392～1.960,0.39～1.960,0.856～4.280,0.424～2.120,1.592～7.960μg,进样质量对数值与峰面积对数值呈良好线性关系;平均回收率分别为98.9%(RSD 3.8%),96.3%(RSD 2.7%),97.7%(RSD 2.6%),97.6%(RSD 3.2%),96.6%(RSD1.9%),102.5%(RSD 2.5%)。结论:建立的含量测定方法简便、准确、分离效果好、重复性好,为更好地控制枇杷叶药材质量提供试验依据。

Objective： To establish an HPLC-ELSD method for simultaneous quantitation of six triterpene acids from Eriobotrya japonica. Method： Samples were analyzed with Ultimate XB C18 column （4.6 mm × 250 mm, 5 μm） and eluted with acetonitrile-methanol-0.5 % ammonium acetate solution as mobile phases in gradient mode, flow rate was 1.0 mL·min^-1, column temperature of 35 ℃ , drift tube temperature of ELSD was set at 95 ℃ , and flow rate of nitrogen was 2.5 L· min ^-1 Result： There were good linear relationship between natural logarithm of peak areas and injection quality at the range of 0. 436-2. 180, 0. 392-1. 960, 0.39-1. 960, 0. 856- 4.280, 0.424-2. 120, 1.592-7.960 μg for rosolic acid, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, ursolic acid, respectively. The average recoveries of these six triterpene acids were 98.9% （RSD 3.8%）, 96.3% （RSD 2.7%）, 97.7% （RSD 2.6%）, 97.6% （RSD 3.2%）, 96.6% （RSD 1.9%）, 102.5% （RSD 2.5%）, respectively. Conclusion： This established method was simple, accurate and repeatable with good separation, it could provide experimental basis for a better quality control of E. japonica.