芪天胶囊中红景天苷和芍药苷的含量测定

HPLC Determination of Salidroside and Paeoniflorin in Compound Qitian Capsules

目的:建立HPLC测定芪天胶囊中红景天苷和芍药苷含量的方法。方法:采用:Kromasil ODS-1色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇(A)-0.02 mol·L-1磷酸(B),梯度洗脱[0～15 min,19%A;15～50 min,19～28%A;50～60min,28%A]分段变波长测定,0～25 min,275 nm(测红景天苷),26～60 min,230 nm(测芍药苷);柱温35℃,流速1.0 mL·min-1。结果:两种成分的线性关系良好,精密度、稳定性、重复性的RSD均<3%,平均加样回收率分别为98.33%,97.77%。结论:该方法简单,灵敏、准确。可用于芪天胶囊的质量控制。

Objective： To describe a method for determining of salidroside and paeoniflorin in compound Qitian capsules by HPLC. Method： The determination was carried out over a COSMOSIL column （4.6 mm × 250 mm,5 μm） at the temperature （35 ℃ ）. The mobile phase consisted of methanol （A） -0. 02% phosphoric （B） solution with the gradient elution （0-15 min, 19% ; 15-50 min, 28% A; 50-60 min, 28% A, the flow rate： 1 mL .min -1 and the detection wavelength was set at 275 nm during 0-25 min and 230 nm during 26-60 min. Result： Two regression equation showed good linear relationship between the peak area ratio of each marker to. internal standard and amounts. The average recovery of salidroside was 98.33% （ RSD 0.71% ） , of paeoniflorin was 97.77% （RSD 1.18%）. Conclusion： The method is simple, accurate, sensitive, and can be used for determination of two components in Qitian capsules with satisfactory accuracy and repeatability.