摘要
目的:建立高效液相色谱法测定地榆配方颗粒中没食子酸的定量分析方法,为制定其质量标准及开发利用提供科学依据。方法:色谱柱:Diamonsil C18(2)柱(4.6 mm×250 mm,5μm),流动相甲醇-0.05%磷酸(5∶95),检测波长272 nm,柱温20℃。结果:在上述色谱条件下,没食子酸进样量在0.009 72~0.097 2 mg·kg-1与其峰面积成良好的线性关系,回归方程Y=34 347.2X+10.887(r=0.999 9);平均加样回收率为99.83%,RSD 2.3%(n=6)。结论:该测定方法简便易行,结果准确,重复性好,可用于地榆配方颗粒中没食子酸的含量测定,并为其提供了有一定参考价值的实验数据。
Objective: To establish the quantitative analysis method for determining the content of gallic acid in Sanguisorba Granule by HPLC so as to provide a scientific basis for development of the drug as well as establishment of its quality standards. Method: Diamonsil C18 (2) column (4.6 mmx 250 ram, 5 Ixm) was used with methanol-0.05% phosphoric acid (5: 95) as the mobile phase; the detection wavelength was 272 nm, the velocity of flow was 1.0mL . min^-1, and the column temperature was 20 ℃. Result: Under the above chromatographic conditions, the injection volume of gallic acid showed a linear relationship with peak area when ranging between 0. 009 72-0. 097 2 mg .kg-1, the corresponding regression equation being Y = 34 347.2X + 10. 887 ( r = 0. 999 9 ) ; the average recovery of gallic acid was 99.83% with corresponding relative standard deviation (RSD) being 2.3% (n = 6). Conclusion: The method is simple, accurate, reproducible and can be used for determination of the content of gallic acid in Sanguisorba Granule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第1期106-108,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技基础条件平台建设项目(桂科0900706)