HPLC测定复方西洋参泡腾片中人参皂苷及维生素C的含量

Content Determination of Ginsenosides and Vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii by HPLC

目的:建立复方西洋参泡腾片中人参皂苷Rg1、Re、Rb1及维生素C的含量测定方法。方法:采用高效液相色谱法,色谱柱为Diamonsil C18柱(4.6 mm×250 mm,5μm),人参皂苷及维生素C的流动相分别为乙腈-0.1%磷酸溶液(梯度洗脱)和甲醇-0.1%磷酸溶液(3∶97),检测波长203 nm和254 nm。结果:人参皂苷Rg1线性范围0.050 4～1.26μg,平均加样回收率98.59%;人参皂苷Re线性范围0.336～8.4μg,平均加样回收率99.11%;人参皂苷Rb1线性范围1.052～26.3μg,平均加样回收率98.12%;维生素C线性范围0.806 4～20.16μg,平均加样回收率97.50%。结论:建立的方法简便、重复性好、准确度高,可用于该产品的质量控制。

Objective： To establish the content determination method for ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and vitamin C in Compound Effervescent Tablets of Radix Panacis Quinquefolii. Method： HPLC method was adopted. The chromatographic column was Diamonsil C18 （4. 6 mm ×250 mm, 5 μm） column. The mobile phases were acetonitrile-0. 1% phosphate solution （gradient elution） and methanol-0. 1% phosphate solution （3 ： 97） respectively. The detection wavelengths were set at 203 nm and 254 nm. Result： The concentration have good linear correlations within the range of 0.050 4-1.26 μg for notoginsenoside Rg1 , 0. 336- 8.4 μg for ginsenoside Re, 1. 052-26.3 μg for notoginsenoside Rb1 and 0. 806 4-20. 16 μg for vitamin C. The average recovery rates were 98.59% , 99. 11%, 98.12% and 97.50% respectively. Conclusion： The method is simple, reproducible and accurate, and applicable for the quality control of the product.