狼毒提取液对小鼠恶性黑色素瘤B16细胞体内转移能力的影响

Effect of Euphorbia fischeriana Extract on Metastatic Ability of Melanoma B16 Cells in vivo

目的:探讨狼毒提取液对小鼠恶性黑色素瘤B16细胞转移能力的影响。方法:分别以荷B16小鼠模型和小鼠恶性黑色素瘤B16细胞实验转移为模型,C57BL/6小鼠随机分为空白对照组、狼毒15.0,20.0 g·kg-1组及环磷酰胺组,检测ip狼毒提取液(EFE)对小鼠B16细胞移植瘤生长和肺转移的影响。免疫组化法分析狼毒提取液对荷B16小鼠移植瘤中抑癌转移因子人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)及促转移因子蛋白激酶B(p-Akt)表达水平的影响。结果:狼毒提取液明显抑制B16细胞的生长,并能显著抑制其肺转移。ip狼毒提取液15.0,20.0 g·kg-1给药14 d,对B16生长的抑制率分别为52.9%,63.6%(环磷酰胺的抑制率为59.5%);对B16细胞肺转移的抑制率分别为37.4%,63.5%(环磷酰胺的抑制率为56.8%)。免疫组织化学法检测结果显示,狼毒提取液组胞质中p-Akt褐黄色颗粒较空白对照组少见,而PTEN胞浆着黄色则较空白对照组多见。结论:狼毒提取液的抗癌转移效果可能部分与其下调促转移因子p-Akt,上调抑癌转移因子PTEN的表达有关。

Objective： To evaluate the effect of Euphorbiafischeriana extract （EFE） on metastatic ability of melanoma B16 cells in vivo. Method： The B16 mouse transplanted tumor model and B16 spontaneous motility melanoma metastatic model were established, and C57BL/6 mice were randomly divided intocontrol group, group EFE 15.0, 20.0 g ·kg^-1 and cyclophosphamide （CTX） group to detect the inhibition effect of EFE for growth of transplanted tumors and metastatic incidence on lung. The expression of phosphatase and tensin homolog deleted on chromosome ten （PTEN） and protein kinase B （p-Akt） of transplanted tumors were observed by immunohistochemical method. Result： EFE could inhibit the growth of B16 cells and the lung metastasis significantly in vivo. The growth inhibition rate of EFE when injected intrapetitoneally to mice at concentration of 15. 0, 20.0 g ·kg^-1 were 52.9% and 63.6% respectively （ cyclophosphamide was 59.5% ） ; inhibition rate of lung metastasis were 37.4% and 63.5% （inhibiton rate of cyclophosphamide was 56.8% ）. Immunohistochemicalresults indicated that compared with control group, expression of p-Akt in EFE group was less apparent while PTEN expression was more visible than control group. Conclusion： EFE could inhibit the metastasis of B16 cells in vivo, probably through down-regulating of p-Akt and up-regulating of PTEN expression, and further study are warranted.