二苯乙烯苷对SAMP8小鼠APP mRNA和PS1 mRNA表达的影响

Effects of TSG on Expression of APP mRNA and PS1 mRNA in SAMP8 Mice

目的:探讨灌胃给二苯乙烯苷(TSG)后SAMP8小鼠β淀粉样蛋白前体(APP)mRNA和早老蛋白-1(PS1)mRNA表达的变化。方法:6月龄快速老化模型小鼠SAMP8 50只,随机均分为SAMP8空白组,阳性药石杉碱甲对照组,高、中、低剂量TSG组(0.3,0.1,0.033 g·kg-1·d-1)共6组;6月龄正常老化小鼠SAMR1鼠10只为正常对照组。各组分别灌胃相应药物60 d后,应用实时定量PCR法检测海马组织APP和早老蛋白-1(PS1)mRNA的表达。结果:与SAMP8空白组比较,TSG组APP mRNA 2-△△CT值明显降低,PS1 mRNA 2-△△CT值也明显降低,差异具有统计学意义(P<0.05),表明TSG组APP mRNA和PS1 mRNA表达下调。结论:二苯乙烯苷对痴呆小鼠SAMP8有显著地抗痴呆作用,其作用机制可能与调控APP,PS1表达有关。

Objective： To observe the effects of 2, 3, 5, 4′-tetrahydroxy-stilbene-2-O-β-D-glycoside （TSG） on expression of β-amyloid precursor protein （APP） mRNA and presenilin-1 （PS1） mRNA in senescence accelerated mouse （SAMP8）. Method： Fifty six-month-old SAMP8 mice were randomized into 5 groups： SAMP8 untreated control group, huperzine A control group, low-, mid-and high-dose groups of TSG （0. 033, 0. 1, 0. 3 g · kg^-1·d^-1）, with 10 mice in each group. Another ten 6-month-old SAMR1 mice were assigned to normal control group. After medication for 60 days, expression of APP and presenilin-1 （PSI） mRNA in hippocampus was assayed by real-time quantitative reverse transcription-PCR. Result： The expression of APP and PS1 mRNA in all dose of TSG groups was down-regulated. Compared with SAMP8 untreated control group, there was significant differences （P 〈 0. 05）. Conclusion： TSG can inhibit the expression of APP mRNA in SAMP8 hippocampus, so as to play the role of anti-senile dementia.