咽舒宁颗粒祛痰镇痛及抑菌作用

Expectorant,Analgesic and Antibacterial Effects of Yanshuning Granules

目的:观察咽舒宁颗粒的祛痰、镇痛、抑菌等药效。方法:将小鼠随机分为对照、阳性药和咽舒宁颗粒高、中、低剂量组(11.00,5.50,2.75 g·kg-1),ig 7 d,采用小鼠气管酚红排泌实验观察其祛痰作用,小鼠热板实验和醋酸扭体实验观察其镇痛作用,采用体外抗菌实验观察其对相关细菌的抑菌作用。结果:咽舒宁颗粒可增加小鼠离体气管酚红排泌量(P<0.05或P<0.01);提高热板所致小鼠的痛阈值(P<0.05);对抗醋酸所致小鼠扭体次数增加(P<0.05或P<0.01);对金黄色葡萄球菌[最小抑菌浓度(MIC)0.250 g·mL-1,最小杀菌浓度(MBC)0.500 g·mL-1]、甲型溶血性链球菌(MIC 0.062 g·mL-1,MBC0.250 g·mL-1)、乙型溶血性链球菌(MIC 0.031 g·mL-1,MBC 0.062 g·mL-1)、变形杆菌(MIC 0.125 g·mL-1,MBC 0.250 g·mL-1)、肺炎链球菌(MIC 0.125 g·mL-1,MBC 0.500 g·mL-1)有较强的体外抑菌作用。结论:咽舒宁颗粒具有明显的祛痰、镇痛和抑菌作用。

Objective： To study the expectorant, analgesic and antibacterial effects of Yanshuning granules. Method： Mice were divided into 6 groups randomly： normal control group, positive groups, high dose group, middle dose group and low dose group of Yanshuning granules （11.00, 5.50, 2.75 g -kg^-1）, after 7 d by ig administration, the expectorant effect was studied by a mouse model of phenol red test. The analgesic effect was studied by mouse models of hot-plate induced pain and acetic acid-induced writhing reaction. Experimental research on antibacterial action in vitro of Yanshuning granules was applied to observe the antibacterial effect. Result： The experimental results showed that Yanshuning granules could significantly increase phenol red excretion in mice （ P 〈 0. 05 or P 〈 0.01 ）. It could significantly prolong the latent time of reaction on hot-plate in mice （ P 〈 0. 05）, reduce the writhing times in mice （P 〈 0.05 or P 〈 0. 01 ）. The granules showed obvious antibacterial actions against Staphylococcus aureus [ minimal inhibitory loncentration （ MIC ） is 0. 250 g · mL^-1, minimal bactericidal concentration （MBC） is 0. 500 g .mL^-1], A-hemolytic streptococcus （MIC is 0. 062 g · mL^-1, MBC is 0. 250 g · mL^-1） , Beta hemolytic streptococcus （ MIC is 0. 031 g · mL^-1, MBC is 0. 062 g · mL^-1 ）, Proteus （ MIC is 0. 125 g · mL^-1, MBC is 0. 250 g · mL^-1） and Streptococcus pneumoniae （MIC is 0. 125 g · mL^-1 , MBC is 0. 500 g · mL^-1）. Conclusion： The granules has obvious expectorant, analgesic and antibacterial effects.