水稻PcG类基因OsEMF2b T-DNA插入杂合突变体的细胞学研究

Cytological studies of PcG gene OsEMF2b T-DNA inserted mutants of rice

采用石蜡切片法对水稻OsEMF2b的T-DNA插入杂合突变体的花器官形态、雌雄蕊发育和种子形成等过程进行了细胞学研究。结果表明,OsEMF2b杂合突变体花器官形态和各部分的数量有变异,但花药和胚珠发育以及花粉和胚囊的育性正常。OsEMF2b杂合突变体出现一定比例的受精卵不分裂、胚胎发育延迟、胚胎畸形,胚乳游离核不能细胞化和胚乳细胞退化解体等异常现象,从而导致其结实率明显低于野生型。OsEMF2b基因可能在水稻的花器官形成和种子发育过程中具有重要的调控作用。

The rice mutants of OsEMF2b inserted by T-DNA were studied cytologically. The results showed that the morphology and quantities of floral organ in heterozygous rice mutants were altered but the development of anther and ovule was not changed. The fertility of pollen and embryo sac of mutants was normal. Abnormities including non-dividing zygote, delayed embryo, abnormal embryo and syncytial with no cellularization,as well as the dissolution and degeneration of endosperm cell occurred during the development of seed. These abnormities resulted in the decrease of seed setting rate. The study indicated that OsEMF2b played an important role in the development of rice floral and seed.