摘要
以华中地区分布的4种野蔷薇叶片为外植体,对其直接再生的影响因子进行研究,以筛选出不定芽诱导率最高的株系及最优的诱导条件。研究结果显示,4种野蔷薇的不定芽最高诱导率差异显著,野蔷薇2号为78.20%,3号为61.16%,4号为13.00%,1号仅5.93%;野蔷薇2号的田间叶片外植体的不定芽诱导率高于组培苗叶片;基因型、植物生长调节剂及叶片的状态对野蔷薇的直接再生影响显著,选择合适的基因型及培养条件极为重要。
Factors affecting plant regenerations of four rose cultivars （Rosa multi2qora Thunb. ） were studied with leaves harvested directly from fields to further determine the optional culture condi- tion and high frequent regeneration genotypes. Results showed that there was significant difference a- mong the adventitious bud regeneration. The highest one （78. 20%） was from 17. multiflora 2,the sec- ond （61.16%） from R. multiflora 3,the third （13.00%） from R. multiflora 4 and the fourth （5.93%） from R. multiflora. The regeneration rate of leaves harvested directly from field is higher than that of leaves from sterile seedling in vitro culture. The results indicted that genotypes,plant hormones and the source of the explants had significant effect on the adventitious shoots induction. It is important to select suitable genotype and optional condition in vitro tissue culture of R. multiflora species.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2013年第1期29-33,共5页
Journal of Huazhong Agricultural University
基金
国家高新技术研究发展计划("863")项目(2011AA100208)
关键词
野蔷薇
直接再生
基因型
不定芽
外植体
Rosa multiflora
direct regeneration
genotype
adventitious bud
explant
