摘要
目的:从血管生成素(Ang)及酪氨酸激酶受体(Tie)表达方面揭示脑脉通促进老龄大鼠脑缺血/再灌注血管生成的作用机制。方法:将SD雄性老龄大鼠随机分为假手术组、模型组、尼莫地平组、脑脉通组,运用免疫组化和原位杂交等方法测定Ang-1、Ang-2,及其受体Tie-2的蛋白与基因表达。结果:脑脉通组各时间点Ang-1、Ang-2、Tie-2蛋白表达均较模型组增强(P<0.05,P<0.01)。脑脉通组缺血3h及脑缺血/再灌注3d、6d、12dAng-2 mRNA以及各时间点Ang-1 mRNA、Tie-2 mRNA表达水平均较模型组增强(P<0.01)。结论:脑脉通促进老年脑缺血/再灌注微血管生成的机制可能与提高Ang/Tie系统蛋白和基因表达有关。
Objective： To reveal the mechanism of action of Naomaitong promoting angiogenesis after cerebral ischemia/ reperfusion in aged rats through Ang and Tie.Methods： The aged male SD rats were randomly divided into sham operation group,model group,nimodipine group and Naomaitong group.To examine the expression of protein and mRNA of Ang-1,Ang-2,Tie-2 by immunohistochemistry and hybridization in situ,etc.Results： Compared with those in model group,expressions of Ang-1,Ang-2,Tie-2 at different time points in Naomaitong group increased（P0.05,P0.01）.And all time points of Ang-1,Ang-2,Tie-2 mRNA in Naomaitong group increased than those in model group（P0.01）.Conclusion： The mechanism of Naomaitong promote angiogenesis after cerebral ischemia/reperfusion may be related to which enhances the expression of protein and gene of Ang/Tie system.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第1期200-203,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金资助项目(No.30772842
No.30973744)~~
