风湿性疾病湿热证实质的脂质组学研究

Study on substance of damp-heat syndrome of rheumatic disease based on lipidomics

目的:以强直性脊柱炎(AS)和痛风性关节炎(GA)为切入点,对风湿性疾病湿热证实质的脂质组学特征进行研究。方法:采用超高效液相色谱/飞行时间质谱联用技术,对61例AS患者、29例GA患者及44名健康志愿者的血清样本进行脂质组学研究,比较AS湿热证患者、AS非湿热证患者、GA湿热证患者以及健康志愿者血清中与湿热证相关的特异性及共性差异脂质代谢物。结果:AS湿热证组与AS非湿热证组血清脂质指纹图谱存在明显差异,其中差异较明显的脂类代谢物主要是甘油磷脂酰胆碱类(GPC)和甘油三酯类(TG);与健康对照组相比,GA湿热证组和AS湿热证组共同表现的溶血甘油磷脂酰胆碱类(Lyso-GPC)明显下降。结论:部分GPC和TG类代谢物可能为AS湿热证脂质代谢的特异性本质,而Lyso-GPC(18:2)、Lyso-GPC(16:0)和Lyso-GPC(18:1)可能为风湿性疾病湿热证脂质代谢的共性实质,为风湿性疾病湿热证实质的探讨提供了新的依据。

Objective： To study the lipidomics characteristics of Ankylosing Spondylitis（AS） and Gouty Arthritis（GA） for tentatively finding the substance of damp-heat（DH） syndrome of rheumatic disease.Methods： Research was performed by using UPLC-QTOF MS coupling technique in 61 AS patients,29 GA patients with DH syndrome and 44 healthy persons as control.Compared the serum lipid metabolites of AS patients with DH syndrome,AS patients with non-DH syndrome,GA patients with DH syndrome and healthy persons to find out the specificity and commonness lipid metabolites of DH syndrome.Results： Serum lipid fingerprint of AS patient with DH syndrome and AS patients with non-DH syndrome were significantly different,the obvious differences in lipid metabolites were phosphatidylcholine（GPC） and glycerin trilaurate（TG）;Compared with the healthy control group,levels of Lyso-GPC in GA with DH syndrome group and the AS with DH syndrome group had significantly decreased.Conclusion： Some GPC and TG lipid metabolites might be the spcificity substance of AS with DH syndrome,and Lyso-PC（18：2）,Lyso-PC（16：0） and Lyso-PC（18：1） might be the commonness substance of damp-heat syndrome of rheumatic disease.This research provided a new basis for the substance study of rheumatic diseases with DH syndrome.