西部地区濒危药用植物秦艽遗传多样性的RAPD分析

RAPD analyses of genetic diversity of endangered G. macrophylla Pall. in western China

目的:对甘肃、陕西、宁夏、四川及青海5个主产地秦艽的遗传多样性进行分析。方法:用随机扩增多态性DNA标记(RAPD)技术对11个秦艽种群90个个体基因组DNA的遗传多样性进行检测。结果:RAPD 8条引物检测到65条扩增条带,其中有59条是多态的,多态位点百分比达到97.78%。通过聚类分析可将秦艽的11个种群聚为两类,四川和陕西两省4个种群聚为一类;甘肃、宁夏和青海省7个种群聚为一类。结论:甘肃省环县种群、子午岭种群和华亭种群应作为秦艽的重要种群加以优先重点保护。

Objective：The study analyzed the genetic diversity of G.macrophylla Pall.in its native growth places in Gansu,Shanxi,Ningxia,Sichuan and Qinghai provinces.Methods： Detect the genetic diversity of the DNA of 90 G.macrophylla Palls from 11 populations through RAPD technique.Results： 65 expansion bands with 59 being polymorphism were detected through 8 primers.Polymorphism rate is 97.78%.The 11 populations were classified into 2 groups through classification analyses,with 4 populations from Sichuan and Shanxi provinces into one group,and 7 populations from Ganshu,Ningxia and Qinghai provinces into another group.Conclusion： The populations in Huan,Ziwuling,and Huating Counties of Gansu Province should be protected with priority as the important populations.