铁皮石斛愈伤组织诱导研究

Study on the Callus Induction of Dendrobium candidum

研究了8种不同浓度的2,4-D培养基和3种不同的培养条件对铁皮石斛愈伤组织诱导的影响。结果表明:诱导愈伤组织时,培养基以0.5 mg/L 6-BA+0.5 mg/L NAA及1.5 mg/L 2,4-D+20.0 g/L蔗糖+200.0 g/L马铃薯泥+2.0 g/L活性碳+10.0 g/L琼脂的培养基效果最好,在暗培养15 d后光培养的条件下诱导愈伤组织效果最好。

The effect of 8 different consistence of 2,4-D culture mediums and 3 different cultivate condition on callus induction of Dendrobium candidum was conducted.The result indicated that the optimal inducing medium was 1/2 MS＋ 0.5 mg/L 6-BA＋0.5 mg/L NAA＋1.5 mg/L 2,4-D＋20.0 g/L sugar＋200.0 g/L mashed potatoes＋2.0 g/L activated carbon＋10.0 g/L agar.The best condition of culture was 15 days in dark then light cultivation.