混合人血清诱发豚鼠过敏反应死亡模型的改良

The Improvement of Mixed Human Serum -induced Anaphylactic Reaction Death Model in Guinea Pigs

目的提高豚鼠过敏反应死亡的发生率和血清肥大细胞类胰蛋白酶检出水平。方法 74只清洁级豚鼠随机分为原模型组、原模型对照组、改良模型组、改良模型对照组和改良模型非致敏组。混合人血清作为过敏原,对注射方式、致敏、发敏方式进行改良,ELISA法测定各组豚鼠血清肥大细胞类胰蛋白酶和总IgE含量。结果原模型组过敏反应死亡发生率为54.2%,豚鼠不同程度心包积血,原模型组及其对照组共有9只因心脏压塞死亡;改良模型组过敏反应死亡发生率为75%,豚鼠无心脏压塞。原模型组血清总IgE含量与其对照组相比差异无统计学意义(P>0.05),但其肥大细胞类胰蛋白酶含量较其对照组升高(P<0.05);改良模型组血清中总IgE和肥大细胞类胰蛋白酶含量均较其对照组升高(P<0.05)。结论改良模型的过敏反应死亡发生率显著提高,为研究血清总IgE和血清肥大细胞类胰蛋白提供了更有效的实验动物模型。

Objective To increase the death rate of fatal anaphylaxis in guinea pigs and the detectable level of the tryptase of mast cell in blood serum. Methods Seventy-four guinea pigs were randomly divided into five groups： original model group, original model control group, improved model group, improved model control group, improved model with non-anaphylaxis group. Using mixed human serum as the allergen, the way of injection, sensitization and induction were improved. ELISA was used to detect the serum mast cell tryptase and total IgE in guinea pigs of each group. Results The death rate of fatal anaphylaxis in original model group was 54.2% with the different degree of hemopericardium. The severe pericardial tamponade appeared in 9 guinea pigs in original model group and original model control group. The death rate of fatal anaphylaxis in improved model group was 75% without pericardial tamponade. The concentra- tion of the serum total IgE showed no statistically difference between original model group and original model control group （P〉0.05）, but the serum mast cell tryptase level was higher in the original model group than that in the original model control group （P〈0.05）. The concentration of the serum total IgE and the serum mast cell tryptase level were significantly higher in improved model group than that in the improved model control group （P〈0.05）. Conclusion The death rate of the improved model significantly increases, which can provide effective animal model for the study of serum total IgE and mast cell tryptase.