远程预处理对肺缺血再灌注氧化损伤的影响

IMPACT OF REMOTE PRECONDITIONING ON OXIDATIVE DAMAGE IN LUNG OF RABBITS AFTER ISCHEMIA-REPERFUSION

目的研究远程预处理对兔肺缺血再灌注损伤的氧化应激反应。方法随机将18只日本大耳白兔分为3组(每组6只):假手术组(S组)、远程预处理组(R组)、缺血再灌注组(I/R组)。制备兔肺缺血再灌注损伤模型,实验结束将动物处死,观察肺组织形态学变化,检测肺湿/干重比值(W/D)值、丙二醛(MDA)含量、肺组织髓过氧化物酶(MPO)活性及超氧化物歧化酶(SOD)活性。结果与S组相比,I/R组肺组织W/D值、MPO和MDA表达均明显升高(p<0.05),而R组上述指标高于S组但低于I/R组(p<0.05);与S组相比,I/R组肺组织SOD活性明显降低,R组上述指标低于S组但高于I/R组(p<0.05)。结论远程预处理在肺缺血再灌注时的抗氧化性,可能与其清除氧自由基及增加内源性抗氧化酶活性有关。

Objective To explore the impact of remote preconditioning on oxidative damage in lung of rab- bits after isehemia - reperfusion. Methods Eighteen healthy Japanese long - ear white rabbits were randomly divided into 3 groups （n = 6） ： sham operation group （S group） ; isehemia -reperfusion group （I/R） and remote precondi- tioning group （R group） . The rabbit lung I/R model were built. At the end of the experiment, The animals were killed and the lungs were removed for determination of lung histological change, ratio of lung wet weight to dry weight （W/D） , malondialdehyde （ MDA ） and myeloperoxidase （ MPO ） content, activities of superoxide dismutase （ SOD ）. Results The W/D ratio of lung tissue, contents of MDA and MPO in lung tissue in the I/R group and R group were significantly higher than those in the sham group （p 〈 0. 05 ）, while the indices above in R group were significantly lower than those in the I/R group（p 〈0.05 ） ; The activities of SOD in lung tissue in the I/R group and R group were significantly lower than those in the sham group （p 〈 0.05 ） , while the indices above in R group were significantly higher than those in the I/R group（p 〈 0. 05 ）. The lung histological change also showed that remote preconditioning could relieve lung isehemia - reperfusion injury. Conclusion These results suggest that remote preconditioning has a certain ability of anti - oxidative damage in lung of rabbits after ischemia - reperfusion. Its mechanism involves oxyradical elimination and increase the endogenous antioxidant enzyme activity.