兔应力性骨折过程中骨骼及骨骼肌组织的形态学改变

Morphological changes of skeleton and skeletal muscle of rabbits in the experimental model of stress fracture

目的 以单光子发射计算机断层仪 SPECT作为诊断依据 ,建立兔应力性骨折 (stress fracture,SF)实验模型 ,观察并探讨 SF发生过程中骨骼及骨骼肌形态的变化 .方法 10只兔作为实验组 ,4只作为对照组 .实验组置于模拟实验装置内 ,使动物产生跑跳运动 .每天跑跳有效次数约 30 0次 . 1wk训练 6 d,共 6 wk.于运动不同时间分别取阳性骨组织和腓肠肌组织行病理学观察 .结果 1肉眼可见实验组病变部位骨骼较对照侧突起、肿胀 ,触之较硬 . 2随着运动天数的延长 ,光镜下病变部位骨骼损害加重 . 3连续观察结果发现 ,运动第 2～ 3周肌纤维损伤最为严重 .结论 在 SF发生过程中 ,骨骼肌组织出现损害的日期早于骨组织 ,说明骨骼肌肉的损害很可能是引起

AIM To investigate the morphological changes of skeleton and skeletal muscle of rabbits in the experimental model of stress fracture. METHODS During 6 weeks' test, 4 rabbits as an control group, and 10 rabbits were stimulatedto run or jump in a special device as experimental group. One or 2 rabbits were sacrificed for the determination in a week. Pathological positive changes of the bone were diagnosed by ECT(electronic computerized tomography), and pathological changes of rabbit sural muscle were observed under the microscope. RESULTS ①The bones from experimental groups were stronger than that from the control group. ②The longer the training day the rabbit lasted, the severer the bone pathological changes were. ③Continues observation of sural muscle found that the damage of sural muscle fiber was much severer during 2~3 weeks. CONCLUSION The early occurrence of the injury of sural muscle might contribute to the occurrence of the experimental stress fracture.