四种传代细胞株软琼脂抛锚能力观察

Anchoring ability of 4 passage cells strain in soft-agar

目的 观察传代细胞在软琼脂中生长情况 .方法 将4种细胞与软琼脂混合均匀 ,铺于平皿中 ,置 37℃ ,5 0～ 10 0m L·L- 1 CO2 环境中培养 .观察细胞集落形成情况 .结果 本实验室的五株非洲绿猴肾细胞 Vero- XAYU ,Vero- XAYE,Vero- XAH,Vero- XAJN和 Vero- XAM,三株猫肾细胞 F81 -XAJU,F81 - XAM和 F81 - XAK不呈现致瘤性独立抛锚生长 ;犬肾细胞 MDCK- XAYE,MDCK- XAYU,MDCK- XAJN,MD-CK- XAJA和 MDCK- XAM五株则呈现较低的致瘤性独立抛锚生长 ;两株金黄地鼠肾细胞 BHK2 1 - XAYU和 BHK2 1 - XAM及两株非洲绿猴肾细胞 Vero- XAYU和 Vero-

AIM To observe the growth of passage cells in soft agar. METHODS Four kinds of passage cells were blended with soft agar and cultured at 37℃, 5%～10% CO 2 atmosphere and then the clone forming was observed. RESULTS Of the five strains of Vero XAYU, Vero XAYE, Vero XAH, Vero XAJN and Vero XAM in our laboratory, 3 strains of F 81 XAJU, F 81 XAM,and F 81 XAK grew anchor free without carcinogenesis, but 5 strains of MDCK XAYE,MDCK XAYU,MDCK XAJN,MDCK XAJA,and MDCK XAM grew anchor free with lower carcinogenesis; 2 strains of BHK 21 XAYu,BHK 21 XAM and another 2 strains of Vero XAYU,Vero XAK demonstrated an anchor free growth with high carcinogenesis.