五种动物传代细胞系对裸鼠的致瘤性

Evaluation of neoplastigenesis of 5 types of passage animal cell lines in nude mice

目的 观察 5种动物传代细胞系对裸鼠是否具有致瘤性 .方法 犬肾细胞系 (MDCK)、非洲绿猴肾传代细胞系(Vero)、猫肾细胞系 (F81 )、地鼠肾传代细胞系 (BHK2 1 )和恒河猴肾细胞系 (MA- 10 4)分别培养后 ,选择对数生长期的细胞进行消化、离心、计数后 ,制备细胞悬液 ,接种裸鼠 ,定期观察 ,通过病理组织形态学观察判定结节是否具有肿瘤的特性 .结果 Vero、F81 、MA- 10 4接种裸鼠后全部有肿块形成 ,经病理形态学检查 ,均为炎性组织 ,其致瘤率为零 .MDCK在 30代以内 ,致瘤率为零 ,第 30～ 6 6代致瘤率为 10 %～ 40 % ,BHK2 1 致瘤率为 10 0 % .结论 F81 ,Vero和 MA- 10 4属非致瘤性细胞系 ,MDCK属低致瘤性细胞系 ,但经冻融或超声波裂解后对裸鼠无致瘤性 ,BHK2 1 属致瘤性细胞系 ,其正常细胞经裂解后仍具有致瘤性 。

AIM To identify whether the passage animal cell lines had neoplastigenesis in the nude mice. METHODS MDCK,Vero,F 81 ,BHK 21 and MA 104 cell lines were cultured respectively and harvested during logarithm growth, and then were counted and resuspended sterily before they were inoculated against nude mice. After a period of growth in nudemice, these cells were evaluated for neoplastigenesis by pathoanotomy and pathomorphology. RESULTS All of the nude mice had neoplasms after inoculation by the vero,F 81 , MA 104 passaging cells. The neoplasms were inflammatory tissue by pathological morphology, and their oncogene ratio was zero. While MDCK's oncogene ratio was zero inside the 30 passage, in the 30～60 passage cells, it became 10～40% and BHK's oncogene ratio was 100%. CONCLUSION F 81 ,vero,and MA 104 belong to non oncogenous cells. MDCK is a type of low oncogenous passage cell, but after being freezed melted and cleavaged for 3 times or disposed with supersonic wave for 5 min, no oncogenesis can be observed, However,BHK 21 can produce malignant tumor in nude mice after inoculation subcutaneously 9～21 days and has a stable repeatability.