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RNA干扰技术在农作物害虫防治中的应用 被引量：7

Application of RNAi Technique in Pest Management for Crop
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摘要 RNA干扰(RNAi)技术作为基因沉默的工具,已被广泛应用于农作物害虫防治研究。准确选择靶基因、将dsRNA或siRNA导入昆虫体内、siRNA在昆虫体内的扩增和扩散是RNAi技术应用于农作物害虫防治的基础。应用RNAi技术能有效地保护农作物抵抗害虫危害,在农作物遗传改良进行精准抗虫方面具有重大的应用前景。 RNA interference （RNAi） technique as a tool for gene silencing has been widely used in agri- culture for the management of insect pests. Accurately selecting target genes, transferring dsRNA or siRNA into insects and amplifying and spreading the siRNA withln the insects are the base for the application of RNAi on pest management. The application of RNAi technique could effectively protect crops from insect pests, and it has better prospect in accurate management of agricultural pests.
作者 郭强 范仲学 褚栋 李晓晴
机构地区 山东省农业科学院高新技术研究中心/山东省作物遗传改良与生态生理重点实验室
出处 《山东农业科学》 2012年第12期16-18,23,共4页 Shandong Agricultural Sciences
基金 山东省自然科学基金项目(ZR2010CM033)资助
关键词 RNA干扰 农作物 害虫防治 RNAi Crop Pest management
分类号 S435 [农业科学—农业昆虫与害虫防治]
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参考文献23

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  • 3Mao Y B, Cai W J, Wang J W, et al. Silencing a cotton boll- worm P450 monooxygenase gene by plant - mediated RNAi im- pairs larval tolerance of gossypol [ J ] . Nat. Biotechnol. , 2007, 25 : 1307 - 1313.
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同被引文献93

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山东农业科学
2012年 第12期

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