正交试验优化淫羊藿总黄酮和多糖的分步提取工艺优化

Orthogonal Array Design for the Optimization of Successive Extraction of Total Flavonoids and Polysaccharides from Epimedium brevicornum Maxim

通过对淫羊藿总黄酮和多糖进行分步提取工艺优化,探寻一种提高淫羊藿综合利用率的提取工艺。利用单因素结合正交试验优化淫羊藿总黄酮的超声波辅助提取工艺,并对此工艺所得药渣进行多糖的水煮提取工艺优化。结果表明,总黄酮提取的最优工艺条件为按料液比1:10(g/mL)加入70%乙醇溶液,在60℃提取温度、150W超声功率下提取2次,每次5min,提取效率达4.72%;多糖提取的最优工艺条件为按料液比1:10加入水,在90℃提取温度提取2次,每次30min,提取效率达1.89%。优化后的淫羊藿总黄酮和多糖分步提取工艺操作简单、能耗低、提取效率高、多糖损失率低、较大限度地提高了淫羊藿的综合利用率。

Objective： To optimize process conditions for the extraction of total flavonoids from the whole plant of Epimedium brevicornum Maxim and for further extraction of polysaccharides from the remaining residue. Methods： A two- step sequential extraction procedure was proposed for the extraction of total flavonoids by ultrasonic-assisted extraction and polysaccharides by hot water extraction. Results： The optimum conditions for the extraction of total flavonoids were found to be two extraction cycles at 60 ℃ and a microwave power of 150 W for 5 min each cycle with 70% ethanol at a solid-to- solvent ratio of 1：10 （g/mL）, resulting in an extraction efficiency of 4.72%. The optimum conditions for the extraction of polysaccharides were found to be two extraction cycles at 90 ℃ for 30 min each cycle with a residue-to-water ratio of 1：10 g/mL, resulting in an extraction efficiency of 1.89%. The optimized procedure for sequential extraction of total flavonoids and polysaccharides was characterized by easy operation, low energy consumption, high extraction efficiency, low polysaccharide loss and enhanced comprehensive utilization ofEpimedium brevicornum Maxim.