β-环糊精包合法纯化紫苏籽油中的α-亚麻酸

Optimization of β-Cyclodextrin Inclusion Conditions to Concentrate α-Linolenic Acid Content in Perilla frutescens Seed Oil

研究利用微射流均质机强化β-环糊精包合紫苏籽油纯化α-亚麻酸工艺,在单因素试验的基础上,选取用料比、处理压力、包合时间3因素进行Box-Behnken试验设计,并利用响应面法对包合工艺进行优化。优化结果表明,在用料比(紫苏油:β-环糊精)1:11(mL/g)、处理压力6000psi、包合时间105min时,β-环糊精包合紫苏籽油的包合率最高达81.64%。利用FT-NIR、扫描电镜对包合物进行物理特性表征,结果证明β-环糊精能够很好的包合紫苏籽油。GC-MS检测表明包合前后的α-亚麻酸的含量由53.50%提高到77.42%,因此表明β-环糊精包合法纯化紫苏籽油中α-亚麻酸工艺可行。

The preparation of inclusion complexes of Perilla frutescens seed oil with β-cyclodextrin using a microfluidizer to increase the α-linolenic acid content in the oil was studied. Response surface methodology based on a Box-Behnken design was used to optimize Perilla frutescens seed oil-to- β-cyclodextrin ratio, pressure and inclusion reaction time. The maximum inclusion rate as high as 81.64% was obtained after 105 rain of inclusion reaction between Perillafrutescens seed oil and β-cyclodextrin at a ratio of 1：11 （mL/g） at 6000 psi. FT-NIR analysis and SEM observation showed that Perillafrutescens seed oil was well included by β-cyclodexWin in the inclusion complex obtained. The α-linolenic acid content increased from 53.50% to 77.42% after β-cyclodextrin inclusion as shown by HPLC analysis. Therefore, β-cyclodextrin inclusion is a feasible way to concentrate the α-linolenic acid content in Perillafrutescens seed oil.