传染性支气管炎病毒实时荧光PCR检测方法的建立

Development of a Real-Time Fluorescent RT-PCR Assay for Detection of Infectious Bronchitis Virus

建立特异、敏感、快速的传染性支气管炎病毒(IBV)实时荧光定量PCR检测方法。针对IBV基因序列保守区域设计一对特异性引物和一条特异性的探针,建立实时荧光定量PCR检测方法,并进行特异性、敏感性、重复性检测。结果表明所建立的IBV实时荧光定量PCR检测方法,引物和探针特异性良好,组间组内重复性良好,检出IBV DNA最小拷贝数为5拷贝。因此,本研究建立IBV特异、敏感、快速的实时荧光PCR检测方法,为IBV的诊断奠定基础。

A real-time PCR method was developed for the specific, sensitive and rapid detection of infectious bronchitis virus （IBV）. A pair of specific primers and a specific probe were designed according to the IBV gene conserved sequence. The specificity, sensitivity and repeatability of this method were analyzed. This method was shown to have good specificity and repeatability and the minimum detection of IBV DNA copy number was 5. This RT-PCR assay showed high IBV specificity, sensitivity and time-saving and thus could provide a helpful tool for the prognosis of IBV.