免疫磁珠法检测脱水蒜制品中沙门氏菌

Detection of Salmonella in Dehydrated Garlic Products with Immunomagnetic Beads

研究免疫磁珠法用于出口脱水蒜制品中沙门氏菌的检测,将免疫磁珠使用于400mL大体积溶液中,并使用高速离心代替磁棒进行分离。致病微生物捕获、分离实验结果表明免疫磁珠法具有特异性强、灵敏度高的特点,沙门氏菌污染量为1CFU/25g时,检出率为70%,污染量为10CFU/25g时,检出率达100%。

Immunomagnetic beads were used to detect Salmonella in dehydrated garlic products. A large volume of Salmonella suspension was added with immunomagnetic beads and high-speed centrifugation was used instead of magnetic bar for bacterial separation. The results of bacterial capture and separation showed that immunomagnetic beads were highly specific and sensitive. The detection rates were 70% and 100% for contamination levels of 1 CFU/25 g and 10 CFU/25 g, respectively.