稀土与葡萄糖氧化酶之间的相互作用

Interaction of Rare Earth with Glucose Oxidase

葡萄糖氧化酶在生物体内是一种重要的大分子,研究稀土元素离子与酶之间的相互作用有利于人们了解稀土元素离子在生物体内的代谢过程和作用机理。本文将葡萄糖氧化酶使用Nafion膜修饰到玻碳电极表面,在分别含有Dy3+,Tm3+,Eu3+稀土的Tris-HCl缓冲溶液中进行了循环伏安以及荧光光谱的研究。首先,考察了三种稀土离子的加入对葡萄糖氧化酶之间的影响。循环伏安测试结果表明,随着Dy3+,Tm3+,Eu3+三种稀土离子的加入,使得葡萄糖氧化酶的氧化还原峰电流逐渐变小,峰电位差变大,这说明电极的可逆性变差,稀土离子的加入引起了蛋白质构象的改变。其次,荧光光谱表明稀土Dy3+,Tm3+,Eu3+对葡萄糖氧化酶有较强的荧光猝灭作用,猝灭机理为静态猝灭。

Glucose oxidase is an important macromolecules in vivo.The interaction between rare earth ions and enzymes was researched to the benefit for metabolic processes and mechanisms of rare earth ions in biological body.In this paper,glucose oxidase was modified on glassy carbon electrode by Nafion membrane.Electrode was studied in the buffer solution containing Dy3＋ orTm3＋ or Eu3＋ rare earth systems by cyclic voltammety and fluorescence spectroscopy.With the addition of three rare earth,the redox peak current of glucose oxidase gradually decreased,peak potential difference was gradually large.The result shown that the electrode reversible became poor and protein conformational changed by rare earth ions.Rare earth was strong fluorescence quenching effect towards glucose oxidase,quenching mechanism is static quenching.