慢性心力衰竭大鼠模型尿液水通道蛋白_2的检测

Urinary excretion of aquaporin_2 water channel protein in rats with chronic heart failure

目的 探讨慢性心力衰竭大鼠模型尿液水通道蛋白2 (aquaporin 2 )浓度的改变及其与肾脏水通道蛋白2 基因表达的相关性。方法 应用左冠状动脉结扎制备慢性心力衰竭大鼠模型 ,术后第 9周应用Western蛋白印迹测定尿液水通道蛋白2 浓度和肾脏水通道蛋白2 表达水平。结果 慢性心力衰竭大鼠和对照组大鼠尿液水通道蛋白2 吸光度比分别为 (36 5 5± 10 2 9) %和 (98 5± 47 6 ) %(P <0 0 1) ,心力衰竭大鼠尿液水通道蛋白2 浓度与肾脏水通道蛋白2 基因表达水平有较好的相关性(r=0 89,P <0 0 1)。结论 慢性心力衰竭大鼠模型尿液水通道蛋白2

Objective To study urinary excretion of aqaporin 2 (AQP2) water channel protein and the relation to renal AQP2 gene expression in rats with chronic heart failure (CHF). Methods Male Sprogue Dawley rats (200 250 g) underwent either a left coronary artery ligation, a model of CHF, or a sham operation. Nine weeks after surgery, urinary AQP2 concentration and renal AQP2 protein level were measured by Western blot. Results The urinary concentration of AQP2 water channel protein was increased significantly in rats with CHF as compared with sham operated rats (365 6%±102 9% vs 98 5%±47 6%, P< 0 01). There was positive correlation between urinary AQP2 concentration and renal AQP2 protein expression ( r =0 89, P< 0 01). Conclusion The urinary concentration of AQP2 water channel protein was increased significantly in rats with chronic heart failure.