麻疯树外植体消毒及愈伤组织的诱导研究

Study on explant germination and callus induction in Jatropha Curcas

为满足麻疯树的大量需求,运用组织培养技术建立麻疯树组培苗的再生体系,以麻疯树叶片或叶柄为外植体,采用不同消毒剂种类和不同消毒时间,研究了麻疯树外植体的消毒以及激素IBA、NAA、6-BA在麻疯树愈伤组织形成中的诱导效果。结果表明,NaClO的消毒效果比HgCl2好,用2%NaClO浸泡外植体20 min为最佳;而NAA比IBA更容易诱导出愈伤组织,较低浓度生长素更适合麻疯树愈伤组织的诱导。麻疯树愈伤组织诱导的最佳培养基为MS+NAA0.1 mg/L+6-BA 1.0 mg/L。

In order to meet the high demand for Jatropha curcas, a regeneration system about tissue culture seedings of J. curcas was established, the leaves and petioles of J, curcas were took as the explants, surface sterilization of explants through the treatment of different disinfectants types and different disinfection time and the induced effect of IBA, NAA, 6-BA to callus formation were studied. The results showed that the sterilization effect of NaClO was better than that of HgCl2, the explants treated in 2% NaClO solution for 20 minutes performed the best. Moreover, compared to IBA, NAA was easier to induce callus formation, and the lower concentration auxin was more suitable for inducing callus. The study illustrated that the best medium of callus formation of 3＇. curcas was MS＋NAA 0.1 mg/L＋ 6-BA 1.0 mg/L.